P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations
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چکیده
منابع مشابه
Resistance to Phosphite Fungicide
There are a number of turf fungicides the activity of which is based on the phosphite (=phosphonate) ion (P03-). Chipco Signature fungicide (active ingredient fosetyl-Al) is the most common, and has become a "keystone" product for disease control programs on many golf courses across the country. This is in part because of the general enhancement in quality on stressed turfgrass that has been re...
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The in situ regeneration of reduced nicotinamide cofactors (NAD(P)H) is necessary for practical synthesis of many important chemicals. Here, we report the engineering of a highly stable and active mutant phosphite dehydrogenase (12x-A176R PTDH) from Pseudomonas stutzeri and evaluation of its potential as an effective NADPH regeneration system in an enzyme membrane reactor. Two practically impor...
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Phosphite dehydrogenase represents a new enzymatic system for regenerating reduced nicotinamide cofactors for industrial biocatalysis. We previously engineered a variant of phosphite dehydrogenase with relaxed cofactor specificity and significantly increased activity and stability. Here we performed one round of random mutagenesis followed by comprehensive saturation mutagenesis to further impr...
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In the title compound, C(4)H(12)N(+)·H(2)PO(3) (-), the components are linked by inter-molecular N-H⋯O and O-H⋯O hydrogen bonds, resulting in a two-dimensional framework.
متن کاملChemical Rescue and Inhibition Studies to Determine the Role of Arg301 in Phosphite Dehydrogenase
Phosphite dehydrogenase (PTDH) catalyzes the NAD(+)-dependent oxidation of phosphite to phosphate. This reaction requires the deprotonation of a water nucleophile for attack on phosphite. A crystal structure was recently solved that identified Arg301 as a potential base given its proximity and orientation to the substrates and a water molecule within the active site. Mutants of this residue sho...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2016
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-016-7993-7